Bioengineering

Introduction to chemical laboratory techniques; qualitative determination of inorganic ions by wet chemical methods; titrations.

• Students know the basics of general and analytical chemistry and have acquired knowledge of qualitative inorganic analysis and basic chemical operations.

• Students will master practical and manual skills of simple chemical analysis, including data processing, presentation, and evaluation.

• Students know the safety equipment in a lab.

Practices; on-the-job training

Final exam: Written work and the assessment of practical work

1. element and compound
2. atomic structure and periodic table
3. chemical bond
4. molecular geometry
5. gases, liquids and solids
6. conservation of mass and energy
7. chemical equilibrium I
8. chemical equilibrium II
9. oxidation and reduction
10. coordination compounds
11. basics of chemical thermodynamics
12. main group chemistry I (hydrogen, alkali metals, alkaline earth metals, noble gases)
13. main group chemistry II (boron group)
14. main group chemistry III (carbon group)
15. main group chemistry IV (nitrogen group)
16. main group chemistry V (oxygen group)
17. main group chemistry VI (halogens)
18. transition metals
19. environmental problems
20. important aspects of general and inorganic chemistry in biological systems

• Students know basics of general, inorganic and physical chemistry.

• Students understand concepts of the anorganic chemistry.

• Students have acquired knowledge about atomic structure, chemical bonds and the different chemical reactions.

• Students know about environmental issues and the essential aspects of bioinorganic chemistry.

• Students know about environmental issues and the essential aspects of bioinorganic chemistry.

Lecture with exercises

Final exam: written exam

- Organic Bonds
- Basic concepts of organic chemistry
- Introduction to organic compounds
- Overview of functional groups, classes of substances and compounds
- Basics of organic reactions

• Students know the properties of organic bonds and basic concepts of organic chemistry.

• Students have basic knowledge of organic compounds, functional groups, substance and compound classes.

• Students know basics of organic reactions.

lecture

Final exam: written exam

• Mathematical methoden in bioengineering (error analysis, dilution, flow rates, centrifugation, interpolation)
• Matrices and linear maps
• Differential calculus and integration, simple differential equations
• Growth and decay
• Linearisation and logarithmic scales

• The students acquire a basic knowledge of mathematics and thus form the basis for the courses in technical and special mathematics, applied statistics, bioinformatics, process engineering and bioprocess engineering.

• The students realise the significance of mathematics in Bioengineering by practically relevant examples.

Lecture with activating, problem-based learning elements.

Final exam: Immanent performance assessment and final written examination

Repetition and strengthening of fundamental mathematical concepts and methods.
In particular, we will focus on the following items.

1. Powers, quantities and units
2. Terms, formulas, logarithm
3. Linear and exponential functions
4. Logarithmic scales
5. Geometry, trigonometric functions, vectors

• The students acquire the basic knowledge in the fields of mathematics and thus forms the basis for the course Technical and Special Mathematics, Applied Statistics, Bioinformatics, Chemical Engineering and Biochemical Engineering, using the same or similar computational examples whenever possible.

• Students get experience and assurance in context with fundamental mathematical procedures

Exercise

Continuous assessment: written assignments

Introduction into microbiology, microbial diversity, microbial growth und cultivation, phenotype and genotype, as well as microbial taxonomy. Selected groups and species with importance to biotechnological production will be discussed in detail. 

• Students understand basic general features of different microorganisms and their phenotype.

• After finishing the lecture students understand microbiological growth processes and their biotechnological use.

• At the end of the lecture, students can connect material cycles and microbial processes like fermentation.

Lecture

Final exam: Final exam, written

Introduction into light microscopy.

Organization, content and function of simple cellular systems. Cellular organization of microorganisms (yeast, bacteria), and plants. Cell wall and organells for higher eukaryotic organisms. Function of plasmatic and non-plasmatic components.

• After completion of the lecture the students are able to work with a light microscope.

• At the end of the lecture, students are able to analyse the functionality of cells, cell components and organs and can interpret this information. Students can communicate their observations and put them in theoretical context.

• Students are able to apply their knowledge to plan simple microbiological experiments.

Lecture and practical exercises

Continuous assessment: Exam, written

Laboratory protocol

Measures and measurement systems, measurement errors, forms and mass of energy and power, the basics of mechanics (force, power, momentum, power transmission, measurement of work, electrical engineering (electric voltage and current, conduction, electric power), heat (basic thermodynamic concepts, measurement of thermal power, main theorems) and optics (geometrical optics, photometry, optics of microscopy).

• Students have basic knowledge of physics and apply it confidently.

• Students master the systematic thinking and approaches of physics and can develop and apply solutions in relevant subfields of physics.

Lecture

Final exam: Written Exam

Introduction to Analytical Chemistry - Fundamentals and Methods; Validation.
Classical quantitative analysis: gravimetry, dimensional analysis (acid-base titrations, precipitation titrations, complexometric titrations, redox titrations).Spectroscopic methods: AAS, AES, RFA, REM; UV/VIS, IR, MS, XPS, NMR.
Electrochemical methods: electrogravimetry, coulometry, conductometry, potentiometry, polarography.
Introduction to separation techniques (chromatography, electrophoresis).

• After successful completion of the course, students have acquired the basics of classical qualitative and quantitative chemical analysis and are thus prepared for the subsequent chemical exercises.

• They have basic knowledge of spectroscopic, electrochemical and chromatographic methods.

This course goes beyond the concept of pure instructional learning (frontal lecture) and expects active listening, i.e. interaction between teacher and student is highly encouraged as time permits.
face-to-face teaching as well as distance learning by means of "zoom
small work assignments between the individual units
presentations by students in small groups

Final exam: written exam

Molar mass / amount of substance, basic laws of stoichiometry, chemical reaction equations, redox reactions, solutions / concentration data / standard solutions, chemical equilibrium / ionic equilibria, gas laws / pH value calculations (acids, alkalis, buffers) / solubilities - solubility product
Volumetry/ Calibration procedures and validation of analytical methods/ Dilutions (ratios)/ Buffers/ Calculations for nutrient media preparation/ Photometric determinations and exercise examples with calibration curves

• After positive completion of the course, students will be able to perform typical tasks related to everyday laboratory work, such as preparing solutions of different concentrations as well as buffers with specific concentrations or pH values on their own.

• They are able to perform the necessary preliminary calculations for the preparation of solutions and the same.

• Students are be able to handle and solve calculation problems as well as laboratory practical problems.

lecture

Lecture on the blackboard and with slides with active involvement of the students, exercises in the lecture, homework, practice examples for independent perfecting.

Final exam: Three written intermediate tests

Data description for a characteristic:
Population and sample, measures, boxplot, frequency distributions, empirical density curves.

Random variable:
Probability theory (probability axioms, addition rule, conditional probability, multiplication rule); discrete random variable (binomial distribution, hypergeometric distribution); continuous random variable (normal distribution).

Parameter estimation:
Estimators, confidence intervals (mean, standard deviation, probability).

Hypothesis testing:
Introduction (alternative, null hypothesis, 1- and 2-sided hypotheses, error, test goodness); 1-sample comparisons ( t-test, binomial test); testing normal distribution assumption (QQ plot, Shapiro-Wilk test); 2-sample comparisons (t-test, F-test); sample size planning (t-test).

Linear regression:
2-dimensional normal distribution, product moment correlation; simple linear regression (least squares estimation, dependence test, coefficient of determination, regression through zero, scale transformations); linear calibration functions.

• Students will be able to solve the following standard statistical tasks: a) are able to describe 1- and 2-dimensional samples with measures and graphs; b) are able to apply the most important rules for calculating probabilities; c) know selected probability distributions to capture the variation of random variables;

• d) are able to estimate distribution parameters based on univariate samples; e) are able to compare means and probabilities within the framework of one-sample comparisons with a given nominal value; f) are able to detect differences between means and proportions in the context of parallel experiments and pairwise comparisons;

• g) Be able to plan minimum sample sizes to detect relevant variances for simple alternative tests; h) be able to perform dependence analyses in the context of simple linear regression models and apply scale transformations to linearize nonlinear dependencies.

lecture and exercise

Final exam: written exam

Within the framework of the chemical-analytical laboratory practical course II, you will carry out quantitative determinations. You will acquire important fundamentals of general and analytical chemistry and basic practical knowledge.

Introduction part (+5 samples)
1st sample: titration and measurement uncertainty
2nd sample: weak acid, alkalimetric
3rd sample: Gravimetric determination of iron
4th sample: Water analysis - determination of water hardness
5th sample: Nitrogen determination according to Parnas-Wagner

• Students will have acquired practical and manual skills of simple chemical analysis, including data processing, presentation and evaluation.

• Students master the practical implementation of the theoretical principles of analytics, especially dimensional analysis and stoichiometry.

practical course

The tasks set are to be processed independently. The "analyses" are to be carried out according to the work instructions. The keeping of laboratory journals and the preparation of reports is practiced. In addition to the work instructions, a script is available in which the theory is also covered.

Final exam: Final testing and assessment of performance in the laboratory

1st sample: Conductometric determination of a salt solution
2nd sample: Potentiometric determination of the equivalent weight of an amino acid (2 students together)
3rd sample: Determination by means of ion-selective electrodes (Cl- next to I-) (2 students together)
4th sample: Photometric determination of iron
5th sample: Photometric determination of the pK value of an indicator (2 students together)
6th sample: Redox titration - vitamin C iodometric
7th sample: Ion chromatographic determination of chloride, nitrate and sulfate (group work)

• Students will have acquired practical and manual skills of simple chemical analysis, including data processing, presentation and evaluation.

• Students are able to practically implement the theoretical principles of analytics, especially dimensional analysis and stoichiometry.

practical course

Final exam: Final written examination and assessment of practical work

1. structural elements of organic compounds (hybrid orbitals, molecular orbitals)
2. reaction mechanisms
3. alkanes (properties, introduction to nomenclature, reactions, preparation, occurrence, representatives)
4. alkenes (isomerism, reactions, representatives)
5. alkynes (representatives, properties, occurrences, reactions)
6. halogen compounds (properties, preparation, reactions)
7. alcohols (properties, preparation, reactions, representatives of monohydric and polyhydric alcohols)
8. ethers (properties, preparation, reactions)
9. sulfur compounds
10. amines (properties, preparation, reactions, representatives), other N- and P-compounds
11. aldehydes and ketones (properties, preparation, reactions, representatives)
12. carboxylic acids (properties, preparation, reactions, representatives)
13. carboxylic acid derivatives (acid halides, esters, amides, anhydrides)
14. amino acids and peptides
15. aromatic compounds (aromaticity, properties, reactions, representatives)
16. dicarboxylic acids
17. hydroxycarboxylic acids
18. organometallic compounds
19. natural products (carbohydrates, fats, oils, waxes, terpenes, nucleic acids)

• Students master the basics of organic chemistry.

• Students will recognize important structures and reactivities as the basis for biochemical processes.

lecture

Final exam: written test at the end

As a basic course of Mechanical Engineering, the following content has to be conveyed:

1) instrcution to technical drawing
2) descriptive geometry (2D, 3D, sectional views, dimensioning)
3) details for manufacturing (tolerances, fits, connections, surface processing, welding symbols)
4) process flowcharts (basic flow chart, process flow diagram, P&ID)

• Studierende können technische Zeichnungen lesen und erstellen.

• Students can create necessary technical specifications for the construction of a machine part or a plant part.

lecture and practise

Continuous assessment: written exam

The course content covers the materials science of the most important metallic (ferrous materials, non-ferrous metals) and non-metallic materials such as ceramic materials, glass, plastics, composites. This area is supplemented by the explanation of corrosion and corrosion protection.
Furthermore, manufacturing processes are taught such as casting and sintering, forging, rolling, pressing, turning, milling, drilling, sawing and grinding, as well as welding, soldering and bonding.
The most important machine elements are taught to complement the material.

• Students have knowledge of the fundamentals and properties of the materials and machine elements used today and can consider their advantages and disadvantages in practical applications. of their advantages and disadvantages in practical use.

• Students are able to make a specific material selection or combination of different materials for plant components and have an overview of the protection of materials.

Lecture

Final exam: written exam

Overview on methods in microbiology as a detailed preparation for the practical courses ('Laborpraktikum')

The methods discussed are: Aseptic techniques, Cultivation of MO, Identification of MO, Determination of growth kinetics, Antibiotics

• Students understand the application of general microbiological knowledge within an industrial setting

• After finishing the lecture, students can translate literature protocols into practical laboratory methods.

• At the end of the lecture, students are able to work safely with biological agents.

Lecture with activating methods

Final exam: Final exam, written

Features of selected production strains (e.g. yeast: Pichia/Saccharomyces; mold: Penicillium/Aspergillus; and bacteria: Bacillus/Lactobacillus) and of important contanminants will be discussed.

This course will present the following aspects of applied microbiology based on selected examples: Microoorganisms in association with Foods; microorganisms in the environment; pharmaceutical indicator organisms; production strains for biopharmaceuticals

• At the end of the lecture, students are able to connect microbial features to industrial applications and products.

• Students can evaluate the utilisation of microorganisms in different biotechnological fields.

• After completion of the lecture, students are able to critically evaluate scientific literature in the field of special microbiology.

Lecture

Distance learning tasks with feedback

Final exam: Final exam, written

Basic concepts of electrical engineering, basic electrical quantities, Ohm's law, electrical switching of loads.
Types of current, line network and electrical connection, electrical installation and connections, protective measures for electrical equipment.
Pictograms on electrical equipment and machines
Electrical drive machines in chemical plants:
Electric motors
Three-phase squirrel-cage motors
Direct current motors
Motor protection types

• Students will have a basic overview and understanding of electrical engineering as it relates to applications in biotechnology.

• Students can read, sketch and calculate electrical diagrams.

Lecture

Final exam: Written Exam

Properties and behavior of fluids, especially aqueous fluids (especially viscosity and surface tension), fundamentals of hydrostatics and hydrodynamics (conservation equations and pipe hydraulics), rheology (sinking velocity of particles, stirrer design) and pump design.

• Students know the basics of hydrostatics and hydrodynamics.

• Students will be able to design piping, agitators, and pumps.

Lecture and exercises

Final exam: Written exam

Mathematical methods play an important role in the applied
Sciences, in particular the following...
- Solving systems of linear equations
- error estimations
- Numerical solution of (nonlinear) equations, zero search
- Interpolation, numerical differentiation
- Integral calculus and numerical integration
The techniques learned are applied manually, but also mechanically (in Python), to solve numerically more complex problems.
In particular, theory from fluid mechanics and hydraulics is used to address applied problems.

• Students possess target-oriented competencies in technical mathematics in direct connection with process engineering. They are thus able to carry out complex problems of technical analysis and process control.

• Students can solve linear and non-linear equations.

• Students master the computer-aided machine solution of numerical problems.

Lecture and exercises

Final exam: immanent performance assessment and final written examination

Introduction into microbiological techniques:
Working under sterile conditions
Different cultivation techniques (surface cultures, liquid cultures)
Cell number determination (according to Koch, Thoma chamber)

Preparation of microbial media

Morphology:
Microscopic imaging of bacteria, yeast and filamentous fungi
Staining techniques. Gram, capsule and spore staining

Physiology:
Growth of yeast on different carbon sources (C-auxanogram)
Antibiotics testing (diffusion test, commercial test stripes, dilution method)
Api test (physiological identification of bacteria with different biochemical tests)

Growth kinetics:
Growth characteristics of E. coli

• Students are able to identify microorganisms

• Students know how to cultivate microorganisms, log and analyse growth curves.

• Students are able to select growth media, generate and sterilise them.

Practical course

Continuous assessment: immanent performance review

1. Material bases of biochemistry: carbohydrates, amino acids, lipids, nucleotides; isomerism, electrolytes.
2. Peptides and Proteins: Structure, function
3. Protein methods at a glance
4. Hemoglobin
5. Enzymes: basics, catalysis, mechanisms, kinetics, inhibition, regulation
6. Intermediary metabolism: basics, energetic considerations
7. Major metabolic pathways:
8. Carbohydrate metabolism: glycolysis, fermentations, citrate cycle, Calvin cycle, pentose phosphate pathway, glycogen
9. Lipid metabolism: fatty acids, cholesterol
10. Biological membranes
11. Respiratory chain and oxidative phosphorylation
12. Amino acid and nucleotide metabolism in overview; cata- and anaplerotic reactions
13. Nitrogen metabolism, urea cycle
14. Photosynthesis
15. DNA and RNA: structure and function
16. Replication, transcription
17. Translation, posttranslational modifications
18. Regulation of gene expression
19. Optional: molecular machines, intracellular sorting of proteins, signal transduction, immune system

• The students know the most essential basics and contents of biochemistry

• Starting from material fundamentals, students master functional aspects of biochemistry and are able to make cross connections to related disciplines (molecular biology, genetics, cell biology, physiology).

• Students will master the major biochemical metabolic pathways such as glycolysis or the citrate cycle.

lecture

Final exam: Intermediate written test and final written exam

Superordinate topics
- Introduction of the UAS laboratory and the equipment in it (precautions and behavior in this regard)
- Structure of scientific texts
- Evaluation of the quality of scientific content
- Scientific literature search
Laboratory methods
- total protein determinations
- Electrophoresis (SDSPAGE)
- Western blot
- ELISA
- Michaelis Menten

• Students will become familiar with the equipment in a laboratory and how to behave properly in a laboratory environment.

• The students will know how scientific texts are structured and how to find scientific literature, and then they will be able to produce scientific texts.

• Students will be able to evaluate the quality of authors of scientific articles, the scientific articles themselves, and the journals in which they are published.

• The students know the laboratory methods they are confronted with in the exercises and can name things like advantages and disadvantages of these methods.

• Students will be able to evaluate data from bioanalytical analyses and assess biopharmaceutical development processes.

lecture; The evaluation of various bioanalytical analyses will be practiced, as well as the assessment of biopharmaceutical development processes.

Final exam: written exam

Overview of basic operations, machines and machine parts used in food and biotechnology, in particular:

    Piping and fittings,
    pipe connections;
    Elements of rotary motion (bearings, seals, joining parts, lid closures, welded and brazed joints),
    Materials: steel, plastics, glass, lubricants; machine science.
    Machines
    - pumps
    - Stirrers, mixers
    - homogenizers
    - centrifuges
    - Machine safety
    Apparatus
    - pressure vessels
    - filter housings
    - heat exchangers
    Water treatment
    - pretreatment
    - filtration process
    - Distillation

Translated with www.DeepL.com/Translator (free version)

• Students apply process engineering principles in practice to combine process units and evaluate them in the process network.

• Students will be competent in the selection, design, specification, and documentation of typical materials, machinery, and equipment used in biotechnology with an emphasis on hygiene and sterile processing.

Lecture

Final exam: written exam

The aim of this course is to provide basic knowledge of cell biology in terms of cellular composition and function. The production (starting from the genetic code) and function of proteins that are essential for proper function of cells and tissue will be discussed in detail. In this respect, an overview of cellular metabolism and energy production as well as storage will be given.
Overall, students that pass this course will have a good understanding of cell biology and molecular biology to succeed in further cell biology and molecular biology classes during their studies.

• Students can name and describe the most important cell organelles and summarize the most important properties of the organelles.

• They know cell therapies based on the T-cell response and can explain complex cellular processes such as the cell cycle.

• They can explain tissue types and know how cells function in a tissue environment.

Lecture

Final exam: written exam

Fundamental operations of mechanical-thermal Process Engineering are discussed in ths lecture. Energy Management and technical Thermodynamics, applied Thermodynamics, mixing & stirring, oxigen transfer, mechanical separating processes, thermal separating processes, physico-chemical separating processes.

• Students know the basic operations of mechanical-thermal process engineering.

• Students will be able to balance processes.

Lecture

Final exam: Written exam

Basic concepts of measurement, principles of electrical measurement of quantities with significance in biotechnological processes.
Basics of sensor elements.
Fundamentals of process automation, process control, in particular, programmable logic controllers (PLCs) and field bus systems.
Introduction to the fundamentals of control theory, types of control systems, analysis, design and simulation of control loops.

• Knowledge of the basics of measurement technology, control and regulation technology, particularly with respect to biotechnological processes.

• Students will be able to select sensors to control biotechnological processes and know how to implement them in higher-level networks.

Lecture with activating elements

Final exam: wirtten exam

Autonomous solving of problems related to the lecture Mechanical-Thermal Process Engineering

• Students are able to develop independent computational approaches to process engineering problems.

Practice

Continuous assessment: immanent performance assessment

Calculation operations of the mechanical-thermal process engineering:

Basic operations of mechanical-thermal process engineering; applied thermodynamics, mass and heat balance, heat transfer, extraction and absorption.

• Students can calculate material and heat balances.

• Students can layout basic mechnical-thermal operation, such as adsoption, extraction, heat transfer, etc.

Pratice

Final exam: written exam

Industrial application of microorganisms and Industrial Bio-Products
Primary and secondary metabolites
Antibiotics
Enzymes and other products

Betalaktams
Biosynthesis of Penicillin and Cephalosporin and strains
Early development
Mould-based Production Technology, productivity and economic constraints
Selected process control parameters
Aspects of Scale-Up
Downstream Processing
Regulatory framework and Summary

• Students will be familiar with industrially relevant cell factories (cells) and different types of biotechnological products (primary, secondary metabolites and recombinant proteins).

• Students understand the use of cells in a process engineering context.

Lecture

Final exam: written exam

Review of elementary concepts and methods (confidence intervals and tests, acceptance sampling, control charts, simulation experiments, design of experiments, regression, calibration experiments, inter laboratory tests.

• After completion of the lecture, students are able to analyse statistical problems within their field with appropriate statistical methods.

• Students are able to select the appropriate statistical method for the task.

• At the end of the lecture, students are able to practically perform the imparted statistical methods.

Lecture, problem based learning

Final exam: Final exam, written

Introduction to the field of Bioinformatics and its applications, specific topics will be discussed in detail, and with practical excercises. Basic concepts of programming will be discussed and illustrated with hands on exercises.

• After completing the lecture students are able to understand basic concepts and algorithms in the field of Bioinformatics.

• Die students can write simple programs.

• After completion of the lecture, students know the most improtant bioinformatics online resources.

Lecutre, discussion and problem based learning

Continuous assessment: Case study and MC-test

Total protein determination, immunological detection methods (ELISA, bead based array, protein arrays, Western blot, ...) interaction analyses (SPR, BLI, ...) proteomics (mass spectrometry, 2 D gel electrophoresis) electrophoresis (SDS PAGE, Blue Native, ...), chromatography, enzymatic analyses, ...

In-depth knowledge of physico-chemical, methodological and instrumental basics of bioanalysis; limits of analytics; data evaluation;

• Students will be able to design bioanalytical studies on production strains and their products and evaluate the results.

• The students master the basic protein analyses such as electrophoresis, chromatography and immunological methods and can name advantages and disadvantages and compare the methods with each other.

• Students are familiar with various interaction analyses and total protein determinations and are also familiar with complex methods such as 2D electrophoresis and mass spectrometry as well as HPLC and other methods.

• Students are able to compare, contrast and differentiate between methods. They can judge when it makes sense to use which method and justify the decision. Ultimately, the students can solve analytical problems by choosing the appropriate methods.

• The students master scientific literature search, and they can present scientific content.

Flipped classroom and problem-based learning in face-to-face unit

Final exam: written exam; presentation with oral exam

Includes as needed and practical: biochemical separation methods, immunochemical and enzymatic methods (e.g. ELISA, Western blot, enzyme analysis), protein analysis, carbohydrate analysis.

• The students can apply the techniques learned in the module Bioanalytics and train the handling of laboratory equipment.

• The students have gained first experience with bioanalytical methods and can evaluate, assess and compare them with each other.

• The students know and master methods that are necessary to follow and evaluate a production process (e.g.: of a monoclonal antibody).

• Students will be able to write scientific texts.

practical exercise

Final exam: Submission of a scientific protocol

Examples from bioprocess engineering are calculated:
- Growth rates
- Substrate consumption/product formation kinetics
- Mass balances for the description of processes
- Evaluation of raw data from the process types batch, fedbatch and chemostat

• Students can calculate problems (calculations) from biotechnical process engineering and are able to conceptualize and calculate plants.

• Students can calculate enzyme and growth kinetics and use them to evaluate biotechnological processes.

Practise

Final exam: immanent performance assessment and final written examination

Brewing Technology:
Raw materials in brewing. Enzymatic processes during malting, mashing, boiling and fermentation&storage of beer. Filling technology in beverage industry.
Analyses and assessment of quality control in breweries. beer design.
beer tasting.

• Students possess the basic skills of brewing and fermentation technology, from the selection of raw materials to process management and quality control. These form the basis of independent activities in the field of brewing and fermentation. Furthermore, through the didactic form of direct experience, they possess an increased understanding of complex industrial biotechnical processes.

• Students will be able to create a beer recipe and can design the brewing process.

Lecture series by several experts, excursion

Final exam: Written exam

Bioprocess engineering includes production systems (cells) and their preservation, support processes such as CIP, sterilization of equipment and media preparation, process types (batch to perfusion), bioreactors and their automation, material and energy transfer (mass and energy transfer).

• Students possess the knowledge of biotechnical process engineering and are thus able to conceptualize and calculate fermentation processes.

• Students will be able to design and operate automated bioreactors for various biological systems.

Blended Learing

Final exam: witten exam

Historical context
Genome, transcriptome and proteome
Differences between eukaryotes, prokaryotes, archaea and viruses.
Replication - Transcription - Translation
Mutations and recombination
structure of DNA; DNA bases; DNA base changes; DNA damage and repair
other methods of DNA analysis

        Sequencing technology
        Agarose gel electrophoresis
        DNA extractions
        Nucleic acid quantification
        PCR / qPCR
        micro arrays
        FISH
        Blotting Techniques
    Transfection, transduction (methods)
    Genome Editing (CRISPR, Zn Finger, ...)
    Plasmid component assembly...
    Expression Hosts
    Cell lineage development

Practical examples

Primer design, mass calculations analytical restriction digestion

• Students possess the theoretical competence of molecular genetics as far as it is necessary for the recombinant development of microbiological strains.

• Specifically, you will be able to transform bacteria and express the desired foreign proteins, e.g. enzymes. This module is directly related to the Molecular Biology Exercises module.

• Students can design primers and can analyze scientific data (e.g.: qPCR) and present this in a text.

lecture

Final exam: Tests at the end

Basic concepts of ethics

• Ethical theories
• Ethics in decision-making and reflection models
• Ethics in everyday life - personal and professional responsibility
• Insights into the ethics committee at Hochschule Campus Wien
• New development trends and their ethical relevance (e.g. AI)
• Ethical issues in relation to the professional field
• current topics in (bio)ethics

• Students are able to understand and explain the central terms and concepts of ethics.

• Students are able to apply ethics to personal and professional responsibility and make ethically sound decisions in everyday life.

• Students are able to critically evaluate, discuss and reflect on important current topics in (bio)ethics, including new technological and social developments such as artificial intelligence, from an ethical perspective.

• Students are able to understand the functioning and purpose of an ethics committee, in particular by gaining insights into the ethics committee of Hochschule Campus Wien.

Lecture with activating methods, group work, discussion, work assignments with (peer) feedback, presentation, reflection, blended learning

Continuous assessment: Immanent performance review

Task:
Insertion of a yeGFP gene into a yeast using CRISPR/CAS9

Creation of a corresponding concept and presentation of this concept with all relevant key data.

• Students have created a reasonable, understandable concept for inserting a gene into a bug.

• Students will be able to communicate the results of their group work in a scientific presentation.

Practical course / seminar

Final exam: Presentation and submission of a concept

Construction of an expression vector to produce l-lactic acid in Saccharomyces cerevisiae
1.) Isolation of the l-lactat-dehydrogenase gen form Lactobacillus plantarum
2.) Construction and amplification of the expression vector in E. coli
3.) Transferring the expression vector in the target organism S. cerevisiae and measurement of the LDH activity

• Students possess detailed skills in molecular genetics that provide them with the foundation to produce and analyze recombinant biomolecules along with the appropriate working techniques.

• Students have learned the common methods and practices of molecular biology based on a defined task.

• Students can write scientific texts.

practical course

Final exam: Submission of a scientific protocol

Content is the brewing process including in-process controls and documentation:

- Recipe design and layout of the brewing process.
- raw material selection
- Execution of the brewing process including documentation
- fermentation monitoring
- Filling, labeling
- IPK (raw materials, chemical and microbial analyses)
- Industrial hygiene and equipment cleaning

• Studierende besitzen die Grundfertigkeiten der Brautechnik von Bierdesign, derAuswahl der Rohstoffe über die Prozessführung bis zur Qualitätskontrolle. Im Weiteren besitzen sie durch die didaktische Form der direkten Erfahrung ein erhöhtes Verständnis komplexer industrieller biotechnischer Prozesse und den Zusammenhang mit dem QM.

• Students master analytical methods of in-process control and can use them correctly in the overall context.

Pratice

Final exam: Final written test and protocol

This course is aiming two major goals:
First of all to develope a common understanding in quality-control-systems concerning the differences to general analytics. Secondly, to deepen the knowledge and background of quality relevant principles and requirements. Methods in breweries quality control, representative for food essentiell quality control will be addressed. Main topics are dealing with laboratory organization, equipment management, Method development and error analysis.

• Students master the basic knowledge and rules of quality control.

• The students have networked the knowledge of several competence areas (quality management, beer analytics, quality control and the fermentation technology laboratory) and understand differences and correlations.

Lecture and discussion

Final exam: immanent performance assessment and final written examination

• Terms, principles, concepts and practice of today's quality management and in particular good manufacturing practice;
• Processes and their procedural instructions;
• Models and standards;
• the concept of "The Deviation" and its general meaning;
• Good manufacturing practice: legal basis and meaning;
• selected aspects of good manufacturing practice.

• Students know the basic concepts of pharmaceutical quality management and understand their significance in the context of GMP. They are able to identify the associated standards and explain their application in practice.

• After completing the course, students are able to critically evaluate the relevance and application of GMP concepts and pharmaceutical QM concepts in various industrial contexts. Students will be able to implement specific GMP requirements in different industrial contexts in order to initiate continuous improvement processes.

Lecture with discussion

Final exam: Written exam

Structure of the ISO 9000ff standards-family
Aim and benefit of quality management systems
Contents of ISO 9001 and the associated standard requirements in detail
Terms related to ISO 9001
Certification of quality management systems based on ISO 9001

• At the end of this lecture, students will understand the requirements of ISO 9001.

• The students know the requirements for a certification according to ISO 9001 and the importance of a certification.

• Students will be able to participate the exam "Certified Quality Representative", which provides an internationally recognized proof of competence in the field of quality management. Note: This certification exam is not part of this lecture.

Lecture and group work, self-study

Continuous assessment: performance evaluation group work, individual oral exam

1. isolation of cells
2. hayflicklimit, telomeres and telomerase
3. specialized cells
4. establishment of continuously growing cell lines (immortalization)
5. tissue engineering, organ culture
6. cell culture laboratory, sterile technique and cryopreservation
7. cultivation methods, cell number, media and additives
8. cell line characterization
9. applications of animal cell lines and development of recombinant cell lines

• Students will have advanced knowledge of applied fermentation and cell technology, especially animal cell technology.

• The students know and understand the central processes in the life of a cell (apoptosis, differentiation, ..).

• Students know how cell lines are produced and how they are characterized and cryopreserved in culture.

lecture

Final exam: written test at the end

Presentation of the final thesis

Examination talk about the final thesis carried out, as well as its cross-references to relevant subjects of the curriculum or a practice-related question and its cross-references to the subjects of the curriculum in the bachelor's degree program.

• Students will be able to present projects.

• Students demonstrate their cross-linked knowledge in biotechnology in a final exam.

Self-study

Final exam: commission examination

The completion of an internship in a company is planned as part of the course of studies.

• Students acquire knowledge of the structures in a specialized company and gain experience in working in an operational environment.

• In addition to direct technical aspects, they learn methods of recording and documentation in practice.

practical application in a professional context

Final exam: written work (written report)

Students describe their activities and experiences during the professional internship they completed.

• Students can reflect on the experiences and knowledge gained during the internship in a structured manner.

• Students may write an internship report.

Practical exercise

Final exam: written work (written report)

The course addresses the technology of GMP-compliant manufacturing aseptically prepared sterile liquid pharmaceutical products and methods to assess the process performance.

• With respect to GMP-compliant manufacturing of medicinal products the students are able to develop a general understanding of aseptic preparation of sterile liquid pharmaceutical products manufactured with different methods.

Lecture and "blended learning" tasks

Final exam: Final exam 100%, "blended learning" tasks are part of the exam material

Aligned to proteins:

1.     Chromatography,
2.     adsorption;
3.     Formulation of proteins as active pharmaceutical ingredients;
4.     Lyophilization;
5.     Reconstitution

• Students understand the principles and methods of isolation and purification of organic molecules, small molecules (metabolites) and polymer molecules (including polysaccharides, but especially proteins).

• Students will know and understand the preparation of these molecules as raw materials for further processing, i.e. crystallization for small molecules and aseptic filling and lyophilization for proteins.

• Students are thus able to use these fundamentals in the subsequent laboratory practical course.

lecture

Final exam: written test at the end

This lecture highlights the process elements of pharmaceutical manufacturing namely personnel, equipment, materials and premises of pharmaceutical manufacturing from the perspective of pharmaceutical process hygiene and contamination.

• Students will recognize hazards posed by contamination and the potential sources of the hazard as it relates to personnel and production areas.

• Students will be able to implement the rule of industrial hygiene.

Lecture and "blended learning" tasks

Final exam: Final Exam 100%, blended learning tasks can compensate up to 5%

Design of a fermentation process
Operation of an automated fermentation plant under aseptic conditions
Practical execution of a fedbatch with E.coli for the production of a recombinant protein
Process evaluation / assessment
Preparation of a protocol

• Students will have the ability to operate automated bioreactors, run aseptic processes, and analyze process data.

• Students master the practical principles of vaccine production.

• Students are proficient in the use of small-scale fermentors and can operate complex computer-controlled technical equipment.

Groupwork

Final exam: Protocol and collaboration

Digitalization is something we encounter more and more often in our daily lives, so the following content will be taught:

    Definition and nomenclature of digitization
    Tools in digitalization
    Project planning for digital processes

The goal of the course is that students learn to know processes with potential for digitization, define the problem in the context of digitization and develop a project plan to convert the process into a digital solution.

• Students can apply the potential of digitization to their field of work.

Lecture with activating methods

Final exam: Presentation, project delivery