Personal Details

In diesem Praktikum wird ein Gen in ein Plasmid eingefügt und es wird anschließend durch verschiedene molekulare Methoden (PCR, Restriktionsverdau) kontrolliert, ob das rekombinante Plasmid in E.coli stabil ist. Vor Beginn des Praktikums erarbeiten die Studierenden in silico Klonkarten für diese Plasmide. Vor Beginn der praktischen Arbeit erfolgt eine Wissensüberprüfung der über Fernlehre vermittelten Inhalte. Im Praktikum wenden die Studierenden Methoden an, die in der Vorlesung Methoden der DNA-Analyse und im Seminar Molekularbiologische & Biophysikalische Methoden theoretisch erarbeitet wurden. Art der Protokollführung: Protokoll in Form einer wissenschaftlichen Publikation - Abstrakt, Einleitung, M&M, Resultate, Diskussion, Literatur.

Aktivierende Methoden

Immanente Leistungsüberprüfung

Vorarbeiten, Antrittsprüfung, Laborarbeit, Protokoll

- Fundamental features of genetics and genetic engineering

- Different levels of regulation of gene expression in pro- and eukaryotes

- Transcriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors, methods for analysis of transcription factors and regulatory regions)

- Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)

- Effects of chromatin (composition, histone modifications, regulation, epigenetics)

- - Methods for analysis of genetics and gene regulation in vitro and in vivo

Lecture

Endprüfung

Written exam on the computer (multiple choice)

- Course Focus: Strategies of scientific research

- Learning Activities:

- Develop a research strategy on a specific topic in drug discovery.

- Design experiments and work packages.

- Evaluate designs based on criteria such as:

- Significance for the research question

- Costs

- Effort

- Final Task: Present the research project as a group.

Dialogue and discussion

Immanente Leistungsüberprüfung

The presentation and discussion of research projects will be evaluated.

- Application of methods for the manipulation and the analysis of signalling pathways in cell culture, leading to a detailed knowledge of specific pathways.

- Applied methods are:

- transient transfection in cell culture,

- reporter constructs with GFP and luciferase,

- overexpression of activators/repressors (including RNAi),

- Western analysis of cellular extracts,

- analysis of phosphorylation,

- fluorescence microscopy of labelled proteins and

- pharmacologic manipulation of the pathways.

Practical course with independent performance of the experiments.

Immanente Leistungsüberprüfung

Entry exam on the computer (multiple choice), evaluation of performance in the lab, protocol

- Important signalling pathways of the cell (e.g. MAP kinase-, GPCR-, Nuclear Hormone Receptor-, NF-kB-, Jak/Stat-, Wnt-, Hedgehog-, Tgfß-, Apoptosis-, PI3K/Akt- and stress pathways)

- Signalling pathways effects on gene expression and other functions of the cell.

- In addition, crosslinks with other signalling pathways are discussed.

- Techniques for the analysis of signalling pathways are also presented.

Lecture

Endprüfung

Written exam on the computer (multiple choice)